Application of inductively coupled plasma-mass spectrometry (ICP-MS) and quality assurance to study the incorporation of strontium into bone, bone marrow, and teeth of dogs after one month of treatment with strontium malonate

Journal article

Authors/Editors

Andersen, Jens

Research Areas

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Publication Details

Author list: Raffalt AC, Andersen JET, Christgau S

Publisher: Springer

Place: HEIDELBERG

Publication year: 2008

Journal: Analytical and Bioanalytical Chemistry (1618-2642)

Journal acronym: ANAL BIOANAL CHEM

Volume number: 391

Issue number: 6

Start page: 2199

End page: 2207

Number of pages: 9

ISSN: 1618-2642

eISSN: 1618-2650

Languages: English-Great Britain (EN-GB)

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Abstract

The strontium content of serum, bone, marrow, and teeth was determined by inductively-coupled plasma mass spectrometry (ICP-MS). Significant correlations were obtained after the data were subjected to quality assurance (QA) performed according to validated procedures. After four weeks of treatment with strontium malonate, strontium levels increased from 76 +/- 9 mu g g(-1) in placebo-treated dogs to levels of 7.2 +/- 1.7 mg g(-1), 9.5 +/- 2.7 mg g(-1), and 9.8 +/- 2.7 mg g(-1) in groups treated with 300, 1000, and 3000 mg kg(-1) day(-1), respectively. Strontium induced a highly significant increase in the bone formation marker, bone-specific alkaline phosphatase (BSAP), and an excellent correlation was found with the bone-strontium content. In females, the placebo-treated group showed a decrease in BSAP of 53%, whereas the three strontium malonate-treated groups showed an increase of 60, 276, and 278% for the groups treated with 300, 1000, and 3000 mg kg(-1) day(-1), respectively. For males the corresponding values were -44%, +142%, +194%, and +247% increases in BSAP in the placebo, 300, 1000, and 3000 mg kg(-1) day(-1) groups respectively.

Keywords

chemometrics/statistics, mass spectrometry/ICP-MS, pharmaceuticals, Quality assurance

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