CYTOCHROME-C DYNAMICS AT GOLD AND GLASSY-CARBON SURFACES MONITORED BY IN-SITU SCANNING TUNNEL MICROSCOPY

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Publication Details

Author list: ANDERSEN JET, MOLLER P, PEDERSEN MV, ULSTRUP J

Publisher: Elsevier

Place: AMSTERDAM

Publication year: 1995

Journal: Surface Science (0039-6028)

Journal acronym: SURF SCI

Volume number: 325

Issue number: 1-2

Start page: 193

End page: 205

Number of pages: 13

ISSN: 0039-6028

eISSN: 1879-2758

Languages: English-Great Britain (EN-GB)

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Abstract

We have investigated the absorption of cytochrome c on gold and glassy carbon substrates by in situ scanning tunnel microscopy under potentiostatic control of both substrate and tip. Low ionic strength and potential ranges where no Faradaic current flows were used. Cyt c aggregates into flat composite structures of about 50 nm lateral extension at gold surfaces. The aggregates evolve in time, and structures resembling individual cyt c molecules can be distinguished in the space between the 50 nm structures. Cyt c aggregates also form at glassy carbon but have a different, unbroken character where cyt c both sticks well to the surface and exhibits notable mobility. The observations suggest that characteristic surface specific, internally mobile protein aggregates are formed at both surfaces and that in situ molecular resolution of the STM pictures may have been achieved.

Keywords

BIOLOGICAL MOLECULES - PROTEINS, POLYCRYSTALLINE SURFACES, scanning tunneling microscopy, SOLID-LIQUID INTERFACES, SURFACE STRUCTURE, MORPHOLOGY, ROUGHNESS, AND TOPOGRAPHY, TUNNELING

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