Capacitive sensing of microcystin variants of Microcystis aeruginosa using a gold immunoelectrode modified with antibodies, gold nanoparticles and polytyramine

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Publication Details

Author list: Lebogang L, Mattiasson B, Hedstrom M

Publisher: Springer Verlag (Germany)

Place: WIEN

Publication year: 2014

Journal: Microchimica Acta (0026-3672)

Journal acronym: MICROCHIM ACTA

Volume number: 181

Issue number: 9-10

Start page: 1009

End page: 1017

Number of pages: 9

ISSN: 0026-3672

eISSN: 1436-5073

Languages: English-Great Britain (EN-GB)

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Abstract

We report on the application of an automated and easy-to-use device to directly measure the immunoreactions between adda-specific monoclonal antibodies and microcystins. The antibodies were immobilized on a gold electrode whose surface was modified first with polytyramine and then with gold nanoparticles. The immunoreaction leads to a change in the capacitance of the system. Under optimum conditions, the sensor is capable of performing stable regeneration-assay cycles and has a low detection limit at a concentration of 0.01 pM level of microcystin-leucine-arginine (MC-LR). The surface of the biosensor can be regenerated with pH 2.5 glycine buffer which dissociates the antibody-antigen complex. The biosensor was used to monitor the production of microcystins during batch cultivation of Microcystis aeruginosa (isolated from ponds in Botswana). Liquid chromatography coupled to MS/MS detection was used to identify three variants, viz. MC-LR (995.6 Da), DmMC-LR (981.2 Da) and MC-LA (910.5 Da).

Keywords

Adda-specific antibodies, Capacitive biosensor, M. aeruginosa, Microcystins, Real-time analysis

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