Tylosema esculentum extractives and their bioactivity

Journal article

Authors/Editors

Research Areas

No matching items found.

Publication Details

Author list: Mazimba O, Majinda RRT, Modibedi C, Masesane IB, Cencic A, Chingwaru W

Publisher: Elsevier

Place: OXFORD

Publication year: 2011

Journal: Bioorganic & Medicinal Chemistry (0968-0896)

Journal acronym: BIOORGAN MED CHEM

Volume number: 19

Issue number: 17

Start page: 5225

End page: 5230

Number of pages: 6

ISSN: 0968-0896

Languages: English-Great Britain (EN-GB)

View in Web of Science | View on publisher site | View citing articles in Web of Science

Abstract

The investigation of Tylosema esculentum (Morama) husks, cotyledons, and tuber yielded griffonilide 2, compound 1, griffonin 3, gallic acid 4, protocatechuic acid 5, beta-sitosterol 6, behenic acid 7, oleic acid 8, sucrose 9, 2-O-ethyl-alpha-D-glucopyranoside 10, kaempferol 11 and kaempferol-3-O-beta-D-glucopyranoside 12. The structures of the isolates were determined by NMR, HR-TOF EIMS, IR and UV-vis spectroscopy, and by comparison with literature data. The husk EtOAc and n-butanol extracts demonstrated >90% DPPH radical scavenging activity at concentrations of 25, 50 and 250 mu g/mL. Furthermore the husk extracts showed higher total phenolic content (233 mg GAE/g). The extractives exhibited minimum inhibitory quantities of 50-100 mu g or no activity against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa and Candida albicans. The tuber extracts were inactive against Caco-2 and Hela cell lines, while the husk extracts showed low activity against Caco-2 and Vero cell line with IC(50) values >400 mu g/mL. The GC-MS analysis showed the beans and tuber non-polar (n-hexane) extracts major constituents as fatty acids. (C) 2011 Elsevier Ltd. All rights reserved.

Keywords

antimicrobial, Antioxidant, Cytotoxicity, Fatty acids, Tylosema esculentum

Documents

No matching items found.