A comparison of flocked swabs and traditional swabs, using multiplex real-time PCR for detection of common gastroenteritis pathogens in Botswana.
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Author list: Mokomane M, Kasvosve I, Gaseitsiwe S, Steenhoff AP, Pernica JM, Lechiile K, Luinstra K, Smieja M, Goldfarb DM
Publication year: 2016
Journal: Diagnostic Microbiology and Infectious Disease (0732-8893)
Journal acronym: Diagn Microbiol Infect Dis
Volume number: 86
Issue number: 2
Start page: 141
End page: 3
ISSN: 0732-8893
eISSN: 1879-0070
Languages: English-Great Britain (EN-GB)
Abstract
We compared the performance of flocked and matched traditional rectal swabs collected from 236 children admitted with gastroenteritis in Botswana. All samples were tested using real time multiplex-PCR assays for nine enteric pathogens. There was a 20% higher detection of Shigella from flocked swabs, but most other pathogens had similar detection rates.
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